Featured Technologies   View all Featured Technologies Methods for Conjugation of Oligosaccharides or Polysaccharides to Protein Carriers Through Oxime Linkages Via 3-Deoxy-D-Manno-Octulsonic Acid Description of Technology: This technology comprises new methods for the conjugation of O-specific polysaccharides/oligosaccharides (O-SP/OS) derived from bacterial lipooligosaccharides/ lipopolysaccharides (LOS/LPS), after their cleavage from Lipid A, to carrier proteins, to serve as potential vaccines. Conjugation is performed between the carbonyl group on the terminal reducing end of the saccharide and the aminooxy group of a bifunctional linker bound further to the protein. The inventors have carried out the reaction under mild conditions and in a short time resulting in binding 3-deoxy-D-manno-octulosonic acid (KDO) on the sacchride to the protein. These conjugates preserve the external non-reducing end of the sacchride, are recognized by antisera, and induce immune responses in mice to both conjugate components (i.e., the OS and the associated carrier protein). Application: Cost effective and efficient manufacturing of conjugate vaccines. Inventors: Joanna Kubler-Kielb (NICHD), Vince Pozsgay (NICHD), Gil Ben-Menachem (NICHD), Rachel Schneerson (NICHD), et al. Patent Status: DHHS Reference No. E-183-2005/0 -- U.S. Provisional Application No. 60/832,448 filed 21 Jul 2006 PCT Patent Application No. PCT/US2007/016373 filed 18 Jul 2007, which published as WO 2008/013735 on 31 Jan 2008 Licensing Status: Available for exclusive or non-exclusive licensing. Portfolios: Infectious Diseases - Vaccines, parasite Infectious Diseases - Vaccines, bacterial Infectious Diseases - Vaccines, other For additional information, please contact: Peter A. Soukas, J.D. Office of Technology Transfer National Institutes of Health 6011 Executive Boulevard, Suite 325 Rockville MD 20852 Phone: 301/435-4646 Fax: 301/402-0220 Email: soukasp@mail.nih.gov